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International Journal of Science, Strategic Management and Technology

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OPTIMIZATION AND MOLECULAR CHARACTERIZATION OF INDOLE ACETIC ACID PRODUCTION BY STREPTOMYCES SPP. ISOLATED FROM RHIZOSPHERIC SOIL

AUTHORS:
Dr Amrita Singh
Mentor
Affiliation
Biochemistry department, govt home science and science college, Jabalpur, India
DOI: 10.55041/ijsmt.v2i3.150
CC BY 4.0 License:
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Abstract

Indole-3-acetic acid (IAA) is a vital plant hormone that significantly influences plant growth and development. The present investigation was designed to isolate Streptomyces species from rhizospheric soil and evaluate their ability to synthesize IAA. Selected isolates were screened using the Salkowski colorimetric method. Further, different cultural conditions such as pH, temperature, and L-tryptophan concentration were optimized to enhance IAA production. The most efficient isolate was subjected to molecular identification through 16S rRNA gene sequencing. The findings indicated that optimized conditions resulted in a marked increase in IAA yield. Molecular analysis confirmed the identity of the isolate as a member of Streptomyces. The study suggests the potential application of these strains in sustainable agricultural practices.

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PUBLICATION DETAILS
Review Type:
Double-Blind Peer Review
License:
CC BY-NC 4.0
Volume/Issue:
Volume 02, Issue 03
Review Rounds:
2
Article Type:
Research Article
Publication Date:
Mar 20 2026
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Singh, D. A. (2026). Optimization and Molecular Characterization of Indole Acetic Acid Production by Streptomyces Spp. Isolated from Rhizospheric Soil. International Journal of Science, Strategic Management and Technology, 02(03). https://doi.org/10.55041/ijsmt.v2i3.150

Singh, Dr. "Optimization and Molecular Characterization of Indole Acetic Acid Production by Streptomyces Spp. Isolated from Rhizospheric Soil." International Journal of Science, Strategic Management and Technology, vol. 02, no. 03, 2026, pp. . doi:https://doi.org/10.55041/ijsmt.v2i3.150.

Singh, Dr. "Optimization and Molecular Characterization of Indole Acetic Acid Production by Streptomyces Spp. Isolated from Rhizospheric Soil." International Journal of Science, Strategic Management and Technology 02, no. 03 (2026). https://doi.org/https://doi.org/10.55041/ijsmt.v2i3.150.

References
1] C. L. Patten and B. R. Glick, "Role of bacterial indoleacetic acid in the development of the host plant root system," Applied and Environmental Microbiology, vol. 68, no. 8, pp. 3795–3801, 2002.

[2] R. Jog et al., "Mechanism and functional diversity of exopolysaccharides from plant-associated Streptomyces," Current Microbiology, vol. 69, pp. 398–403, 2014.

[3] S. Spaepen, J. Vanderleyden, and R. Remans, "Indole-3-acetic acid in microbial and microorganism-plant signalling," FEMS Microbiology Reviews, vol. 31, pp. 425–448, 2007.

[4] J. E. Clarridge, "Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases," Clinical Microbiology Reviews, vol. 17, no. 4, pp. 840–862, 2004.

[5] S. A. Gordon and R. P. Weber, "Colorimetric estimation of indoleacetic acid," Plant Physiology, vol. 26, pp. 192–195, 1951.

[6] S. Khamna, A. Yokota, and S. Lumyong, "IAA production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils," Journal of General and Applied Microbiology, vol. 56, pp. 195–202, 2010.

[7] J. M. Janda and S. L. Abbott, "16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls," Journal of Clinical Microbiology, vol. 45, no. 9, pp. 2761–2764, 2007.
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This article has undergone plagiarism screening and double-blind peer review. Editorial policies have been followed. Authors retain copyright under CC BY-NC 4.0 license. The research complies with ethical standards and institutional guidelines.
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