HIGH FREQUENCY INDUCTION OF CALLUS FROM SEEDLING EXPLANTS OF PIGEONPEA
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A simple, efficient, and reproducible protocol for high-frequency callus induction was established using leaf, hypocotyl, and embryo explants excised from 12-day-old seedlings of pigeonpea genotypes GT 101 and UPAS 120. Callus induction was observed at varying frequencies across different combinations of 2,4-D and BAP. Initiation of callus was evident from the cut surfaces of explants after a mean period of 10.4 days of inoculation. The highest callus induction frequency (85%) was recorded on MS (Murashige and Skoog) medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP when hypocotyl explants were used.
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Hitesh, J., Nimita, U. & D, P. L. (2026). High Frequency Induction of Callus from Seedling Explants of Pigeonpea. International Journal of Science, Strategic Management and Technology, 02(04). https://doi.org/10.55041/ijsmt.v2i4.459
Hitesh, Jasani, et al.. "High Frequency Induction of Callus from Seedling Explants of Pigeonpea." International Journal of Science, Strategic Management and Technology, vol. 02, no. 04, 2026, pp. . doi:https://doi.org/10.55041/ijsmt.v2i4.459.
Hitesh, Jasani,Umretiya Nimita, and Parmar D. "High Frequency Induction of Callus from Seedling Explants of Pigeonpea." International Journal of Science, Strategic Management and Technology 02, no. 04 (2026). https://doi.org/https://doi.org/10.55041/ijsmt.v2i4.459.
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